Abstract
To achieve high removal of both organic matter and nitrogen, the advanced management of activated sludge process was required. We studied on the simple methods for the determination of organic matter oxidizing and nitrifying activities by measuring oxygen uptake rate (OUR), dehydrogenase activity and ATP (adenosine 5'-triphosphate) activity using specific inhibitors for ammonia and nitrite oxidizing bacteria. The methods were mainly applied to a sequential batch activated sludge process with intermittent aeration treating swine wastewater. The results obtained were summarized as follows: It was possible to determine both organic matter oxidizing and nitrifying activities by measuring OUR, dehydrogenase activity and ATP activity according to our methods. Linear correlations among OUR, dehydrogenase activity and ATP activity related to organic matter oxidation were observed in spite of the origin of activated sludge. Ammonia oxidizing and nitrite oxidizing activities also had similar correlations. It became clear that the relationship between organic matter oxidizing and nitrifying activities was characterized by the supply of oxygen and substrates to activated sludge. Rational management of activated sludge process for the removal of both organic matter and ammonia was considered to be possible by keeping the maximum nitrifying activity.
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