Проточно-инжекционное определение валина пьезокварцевым сенсором, модифицированным полимером с молекулярными отпечатками

Abstract

Flow-injection determination methods of analysis are gaining their popularity due to vast production of pure amino-acids and their quality control. This report describes methods of valine determination in aqueous solutions using a flow-injection system with piezoelectric sensor on the basis of molecular imprinted polymers (MIP) as a detector. To produce MIP polyamide acid was synthesized at 453 K directly on the surface of the sensor. The tamplate was removed without destruction of polymeric coating. Modified sensors MIP-Val were fit into a cell of original design. The speed of the eluent flow through the cell was 0.9 ml/min. The volume of the injected sample was 100 uL. The experimental part proved the ability of piezoelectric sensor modified with molecular imprinted polymer to determine valine in binary mixtures with glutamic acid. Detection limit of valine was 10 -5 mol/L. The obtained data showed selectivity of piezoelectric sensor modified with (MIP) only to valine. Imprinting factor for the sensor modified with MIP-Val, IF =3.2 Keywords : valine, glutamic acid, piezoelectric sensor, molecular imprinted polymers (MIPs), flowing-injection analysis. (Russian) DOI: http://dx.doi.org/10.15826/analitika.2014.18.4.010 *A.N. Zyablov, *S.I. Govorukhin, *O.V. Duvanova, *V.F. Selemenev, **Nguen An' T'en * Voronezh State University , Voronezh, Russia n Federation **Ho Chi Minh City University of Pedagogy, Ho Chi Minh City, Viet Nam REFERENCES 1. Kel'ner R., Merme Zh.-M., Otto M., Vidmer G.M. Analiticheskaia khimiia. Problemy i podkhody [Analytical Chemistry. Issues and Approaches], Moscow, Mir: AST, 2004. Vol. 1. 608 p. (in Russian). 2. Evgen'ev M.I., Garmonov S.Iu., Shakirova L.Sh. [Flow injection analysis of drugs]. Zhurn. analit. khimii [J. of Anal. Chem], 2001, vol. 56, no. 4, pp. 355-366 (in Russian). 3. Gendrikson O.D., Zherdev A.V., Dzantiev B.B. [Molecularly imprinted polymers and their use in the biological assay]. Uspekhi biologicheskoi khimii [Success of biological chemistry], 2006, vol. 46, pp. 149-192 (in Russian). 4. Ziablov A.N., Kalach A.V., Zhibrova Iu.A., Selemenev V.F., D'iakonova O.V. [Determination glycine piezosensor aqueous solutions modified with a molecularly imprinted polymer]. Zhurn. analit. khimii [J. of Anal. Chem], 2010, vol. 65, no. 1, pp. 93-95 (in Russian). 5. Zhibrova Iu.A., Ziablov A.N., Shcheglova N.A., Krasnikova O.P., Selemenev V.F. [Molecularly imprinted polymers for piezoelectric sensors Message 1. Analysis of pharmaceutical preparations containing glycine]. Sorbtsionnye i khromatograficheskie protsessy [Sorption and chromatographic processes], 2008, vol. 8, no. 4, pp. 686-688 (in Russian). 6. Ziablov A. N., Monicheva T.S., Selemenev V.F. [Detection of amino acids in the preparation of "BCAA" piezoelectric sensor modified with molecularly imprinted polymers]. Analitika i kontrol' [Analitics and control], 2012, vol. 16, no. 4, pp. 406-409 (in Russian). 7. Ziablov A.N., Nikitskaia L.M., Zhibrova Iu.A., Kalach A.V., Selemenev V.F. P'ezosensor na osnove polimerov s molekuliarnym otpechatkom aminokislot [Piezosensor based on Molecularly imprinted polymers of amino acids]. Patent RF. no 2010142819/28, 2011 (in Russian). 8. Bykov V.A., Krylov I.A., Manakov M.N. Mikrobiologicheskoe proizvodstvo biologicheski aktivnykh veshchestv i preparatov [Microbiological production of bioactive substances and drugs]. Moscow, High school, 1987. 143 p. (in Russian). 9. Cormack P. A. G., Elorza A. Z. [Molecularly imprinted polymers: synthesis and characterization]. Journal of Chromatography B, 2004, vol. 804, no. 1, pp. 173-182. 10. Dmitrienko S.G., Irkha V.V., Kuznetsova L. Iu, Zolotov Iu. A. [Use of molecularly imprinted polymers in separation processes and concentration of organic compounds]. Zhurn. analit. khimii [J. of Anal. Chem.], 2004, vol. 59, no. 9, pp. 902-913.