Количественная оценка антиадгезивного и антибактериального эффектов биологически активной добавки Уронекст® в отношении уропатогенов: микробиологическое исследование.

Abstract

Introduction. Antibacterial drugs are the basis of pharmacotherapy of lower urinary tract infections (UTI), and there is a growing interest in alternative nonantibacterial methods of their treatment and prevention. Purpose of the study. Quantitative evaluation of the anti-adhesive and antibacterial effects of the dietary supplement «Uronext®» containing D-mannose, vitamin D3 and cranberry extract against uropathogens isolated from patients with recurrent UTI. Materials and Methods. 124 strains of uropathogens isolated from the urine of women with recurrent UTI were studied. At the first stage of the study we determined the sensitivity of microorganisms to Uronext dietary supplement. In its presence, uropathogens were the material for the second stage of the study, during which the minimum inhibitory concentration of «Uronext®», its effect on the adhesion index (AI) of uropathogens, as well as the degree and duration of antimicrobial activity were evaluated. Results. The direct antibacterial effect of «Uronext®» against 53.7% of Gram-negative and 51.7% – of Gram-positive microorganisms strains was revealed. A pronounced anti-adhesive effect of the investigated product was noted, especially against Gram-negative bacteria. The anti-adhesive effect of «Uronext®» was the greatest in the first 4 hours and lasted for the whole 24-hour observation period. The minimum inhibitory concentration of «Uronext®» for Gram-negative bacteria was 8 times lower than for Gram-positive bacteria. Antibacterial activity of «Uronext®» was manifested already 1 hour after the product was added to the cell culture, reached the highest value in 2 hours and remained at the maximum level during 5 hours of observation. The mentioned tendency was characteristic for all investigated microorganisms. Conclusion. The conducted study allowed to establish and quantitatively evaluate the expression of anti-adhesive and antibacterial action of the product «Uronext®» against the main uropathogens. The presence of these effects determines the pathogenetic validity of the prescription of the dietary supplement «Uronext®» to patients with recurrent UTI.