Обезвоживание эритроцитов млекопитающих влияет на их чувствительность к механическому стрессу

Nataliya M Shpakova,Elena Ye Nipot,Nataliya V Orlova

doi:10.15407/cryo25.01.024

Abstract

Sensitivity of human, bovine, rat and equine erythrocytes to effect of mechanical stress in the media containing 0.15, 0.4 and 0.6 mol/l NaCl was studied. Mechanical stress of mammalian erythrocytes in the medium containing 0.15 mol/l NaCl results to the loss of K+ by cells: from 30% for equine erythrocytes and 60% for bovine cells. Bovine and equine erythrocytes in the medium, containing 0.15 mol/l NaCl show a maximum resistance on hemolysis index to mechanical stress effect. It has been shown that dehydration of human, bovine, rat, and equine erythrocytes in the media with 0.4 and 0.6 mol/l NaCl increases sensitivity of cells to mechanical stress. Bovine and rat erythrocytes are the most sensitive to an increase in osmotic load under mechanical stress on hemolytic damage index, and equine cells are the most sensitive to potassium ion outflux index. Different sensitivity of mammalian erythrocytes to mechanical stress under osmotic load is supposed to be determined by specific peculiarities of water transport and lipid composition of erythrocyte membranes. Comparative analysis of dehydration effect of erythrocytes on their resistance to stress factors showed that formation of stable state of cells (in the medium with 0.4 mol/l NaCl) to hypertonic shock effect did not provide their resistance under mechanical stress. Probl Cryobiol Cryomed 2015; 25(1): 24-32.

Highlights

Sensitivity of human, bovine, rat and equine erythrocytes to effect of mechanical stress in the media containing 0.15, 0.4 and 0.6 mol/l NaCl was studied
Incubation of human, rat and equine cells in saline media with relatively low concentrations (0.3–0.4 mol/l NaCl) results in developing of a maximum resistance to the following effect of hypertonic shock [14, 17], i.e. enter the state, which thereinafter will be called as ‘stable’. Whereas this effect has been found for erythrocytes of different mammalian species [17], we suggest a certain versatility of the approach, associated with partial dehydration of cells in appearance of stability of mammalian erythrocytes against hypertonic shock effect
Preliminary dehydration of mammalian erythrocytes increases their sensitivity to mechanical stress (MS) action

Summary

Introduction

Sensitivity of human, bovine, rat and equine erythrocytes to effect of mechanical stress in the media containing 0.15, 0.4 and 0.6 mol/l NaCl was studied. Bovine and equine erythrocytes in the medium, containing 0.15 mol/l NaCl show a maximum resistance on hemolysis index to mechanical stress effect. It has been shown that dehydration of human, bovine, rat, and equine erythrocytes in the media with 0.4 and 0.6 mol/l NaCl increases sensitivity of cells to mechanical stress. Что начальное состояние эритроцитов млекопитающих определяет устойчивость клеток к действию гипертонического шока [2, 7, 9]. После инкубирования клеток человека, крысы и лошади в солевых средах с относительно невысокой концентрацией (0,3– 0,4 моль/л NaCl) они проявляют максимальную устойчивость к последующему действию гипертонического шока [5, 9], т. Механизм повреждения эритроцитов в условиях гипертонического шока также связывают с образованием трансмембранных пор [5, 9], на формирование которых предварительное частичное обезвоживание клеток может оказывать опосредованное влияние

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Conclusion