Abstract
The chlorpromazine effect on sensitivity of human erythrocytes to the action of posthypertonic shock and their resistance at glycerol removal from the cells exposed to freeze-thawing has been studied in our research. It has been shown that under conditions of posthypertonic shock of erythrocytes (at 0°C) the chlorpromazine reduced a hemolysis level in 3.7 times, and in case of 600 µmol/l concentration its maximum antihemolytic activity was 73 ± 6%. The application of chlorpromazine during removal of glycerol from frozen-thawed erythrocytes enabled to reduce the cell damage by 2.5–3 times. The values of antihemolytic activity of chlorpromazine in both cases were quite similar. Results of the investigation of chlorpromazine efficiency in the model experiment and under the real conditions of erythrocyte freeze-thawing (under glycerol removal from the cells) allow to conclude about the applicability of this model (posthypertonic shock) in the further studies. Probl Cryobiol Cryomed 2017; 27(1): 51–60
Highlights
The chlorpromazine effect on sensitivity of human erythrocytes to the action of posthypertonic shock and their resistance at glycerol removal from the cells exposed to freeze-thawing has been studied in our research
Studying the processes occurring at different stages of low-temperature preservation of biological objects is typical for cryobiology [2, 8, 13, 15]
Glycerol was removed from erythrocytes according to the following protocol [17, 22]: the 1st stage represented washing with an equal проблемы криобиологии и криомедицины problems of cryobiology and cryomedicine том/volume 27, No/issue 1, 2017
Summary
The chlorpromazine effect on sensitivity of human erythrocytes to the action of posthypertonic shock and their resistance at glycerol removal from the cells exposed to freeze-thawing has been studied in our research. It has been shown that under conditions of posthypertonic shock of erythrocytes (at 0°C) the chlorpromazine reduced a hemolysis level in 3.7 times, and in case of 600 μmol/L concentration its maximum antihemolytic activity was (73 ± 6)%. Results of the investigation of chlorpromazine efficiency in the model experiment and under the real conditions of erythrocyte freeze-thawing (under glycerol removal from the cells) allow to conclude about the applicability of this model (posthypertonic shock) in the further studies. Studying the processes occurring at different stages of low-temperature preservation of biological objects is typical for cryobiology [2, 8, 13, 15] This is because of a need in considering the mechanisms of cryoinjury and cryoprotection when developing new cryopreservation methods, in contrast to an empirical selection of the media, freezing and thawing conditions for a biological object.
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