Ｇ−ＣＳＦ製剤の持続静注における輸液セットへの吸着

Abstract

The change in the adsorption to infusion sets, the adsorption ratio and the recovery ratio of recombinant human granulocyte-colony stimulating factor (G-CSF) were investigated in vitro by administering a continuous intravenous infusion of G-CSF.After 250μg of G-CSF (nartograstim) preparation was dissolved with 100 ml of sterile saline in a commercially produced polypropylene container, operating the pump (8 ml per hrs), the G-CSF levels in the container, in the upper part of the final filter, in the lower part of the final filter, and in the collecting vessels, were determined by enzyme-linked immunosorbent assay (ELISA) for GCSF at various time (0, 0.5, 1, 1. 5, 2, 3, 6, and 10 hrs)In the first day's experiment, that is, using a new infusion set (container, line and filters), the GCSF levels in the upper and lower part of filter and in the collecting vessels were very low during to 0.5 to 1.5 hrs after operating. However their levels roughly recovered until the container's during 3 to 6 hrs. A marked adsorption of G-CSF was also observed not only into the container and filters but also into the line. Notably the recovery of G-CSF was improved in the second day's experiment.It is necessary for physicians to give consideration to these adsorptions and to the loss of G-CSF when a small amount of G-CSF is dissolved for treatment by continuous intravenous infusion.