義歯床の維持に関与する唾液蛋白質 二次元ポリアクリルアミドゲル電気泳動法によるヒト顎舌下腺唾液の分析

Abstract

The purpose of this study was to examine the role of protein of human submandibular-sublingual saliva in the retention and stability of dentures. This study was performed by analyzing how changes in protein affect viscosity.Submandibular-sublingual saliva samples obtained from 15 subjects, each with a healthy oral cavity and good general condition, were studied. A Kuette type rotary viscometer Low-Shear 30 (Contraves) was used to determine viscosity. The analysis of salivary proteins was achieved by combining microscale twodimensional SDS-PAGE with silver staining.In high viscosity saliva, 44 protein spots were detected. These spots appeared in the range of pI 3.8-10.0 and estimated molecular weight of 15, 000-1, 000, 000. In low viscosity saliva, approximately 30 protein spots were detected. These spots appeared in the range of pI 3.8-10.0 and estimated molecular weight of 15, 000-1, 000, 000.There was a significant correlation between the concentration of high molecular weight proteins with an estimated weight of more than 700, 000 and viscosity (r=071, p<0.01). High molecular weight proteins (with an estimated weight of more than 700, 000) were characterized into five groups to the isoelectric point. The two groups (more than pI 7.0 and pI 4.1-5.1) showed significant correlations with high viscosity of human submandibular-sublingual saliva (r=0.73 and p<001 for both groups).