Abstract
The dissociation constants of proton-acceptor and -donor groups of adrenaline (epinephrine) and mesatone were determined by potentiometric and spectrophotometric titration method. The potentiometric titration data were used to calculate the protonation constants of a secondary amino group, and the spectrophotometric titration data determined the dissociation constants of OH groups influencing the distribution of electron density in the chromophore moiety(aromatic system). Once the dissociation constants are known, the distributions of the ionized forms of epinephrine and mesatone at various pH can be calculated. It these calculations, it is important to take into consideration the transport of biological molecules through membranes and their interactions with receptors.
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