Abstract
Adult T-cell leukemia associated antibody (ATLA-Ab) positive persons were screened by indirect immunofluorescence (IF) testing. and their peripheral blood mononuclear cells (PBMC) collected from concentrated red blood cell (CRC) were cultured in vitro with and without phytohemagglutinin (PHA-P) after the irradiation of 2000·4000 and 8000rad. The appearance of ATL virus positive PBMC during the in vitro culture for 10 days was analyzed by IF assay using mouse monoclonal antibody ATLV-19 reactive to p19 core protein of ATLV. All of 12 samples showed ATLV positive PBMC after the culture for 10 days without irradiation. Irradiated samples decreased the appearance of ATLV positive cells, and 8000rad irradiation failed to induce ATLV positive cells in 10 out of 12 cases. This inhibition of ATLV positive cell induction is well coincident with that of in vitro cell proliferation stimulated by PHA-P.These evidences showed the possibility that the irradiation of blood product is effective for the prevention of ATLV transmission by blood transfusion.
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