血管柄付骨膜とアテロコラーゲンを用いたＰｒｅｆａｂｒｉｃａｔｅｄ ｖａｓｃｕｌａｒｉｚｅｄ ｂｏｎｅ ｇｒａｆｔ作製に関する実験的研究

Abstract

A rat model was used to study a technique for constructing prefabricated vascularized bone grafts using vascularized periosteum, atelocollagen, and bone marrow. The animals were divided into two groups, A and B. Group A received vascularized periosteal grafts combined with atelocollagen, and group B received composite grafts of atelocollagen and bone marrow. Grafts in the controls were prepared only with periosteum. Histological, radiographic, and biochemical observations were made between 3 and 5 days and at 1, 2, 3, 5, 7 and 9 weeks.The results were as follows: Alkaline phosphatase activity appeared in the periosteum 3 days after operation, and osteoid ossification formed from the periosteum was noted 5 days after operation in all groups. Active bone formation occurred in all animals during the 2 weeks after surgery. Grafts in the two experimental groups produced a greater amount of new bone than they did in the controls between 5 and 9 weeks after surgery. Although in group A a larger amount of bone was formed with endochondral and membranous ossification than in group B, which showed membranouse ossification only, the rate of bone formation in group B was faster than that in group A.It was concluded that atelocollagen may serve as an effective carrier of vascularized periosteum and that bone marrow accelerates the growth of bone in the periosteum.