Abstract
Producing large quantities of microalgae is one of the most limiting factors when developing large-scale culture techniques for shellfish. When artificial light is used for producing microalgae, it is one of the most expensive costs. In this study, we conducted experiments to determine the optimum light intensity and efficient culture protocol for microalga Pavlova lutheri using a 200-liter chamber.A 200-liter culture chamber was used to determine the average distribution of PPFD. Under two conditions : 1) filtered seawater (control), and2) filtered seawater with culture medium added and 1.0×107 cells·ml-1 P. luatheri. Average distribution of PPFD for the filtered sea water was 1, 800μmol·m-2·s-1 at irradiation of the full light intensity. But when P. lutheri was cultured in the chamber, available photosynthetic area was reduced to a half of the chamber.P. lutheri was cultured at irradiation of10%, 30%, 60%and full light intensity at 15°C and an aeration of 30l·min-1. Maximum cell density correlated positively to light intensity with full light regime supporting more than 2.0·107cells·ml-1.We conclude that if higher light intensity is used for the chamber, P. lutheri will obtain nigher cell densities. Additionally the control of light intensity is effective method to decrease the production cost of P. lutheri.
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