목질계 셀룰로오스 에탄올 생산공정에서 전처리과정의 설계

Abstract

A pretreatment process of cellulose decomposition to a monosaccharide plays an important role in the cellulosic ethanol production using the lignocellulosic biomass. In this study, a cellulosic ethanol was produced by using acidic hydrolysis and enzymatic saccharification process from the lignocellulosic biomass such as rice straw, sawdust, copying paper and newspaper. Three different pretreatment processes were compared; the acidic hydrolysis (100 ℃, 1 h) using 10~30 wt% of sulfuric acid, the enzymatic saccharification (30 min) using celluclast (55 ℃, pH = 5.0), AMG (60 ℃, pH = 4.5), and spirizyme (60 ℃, pH = 4.2) and also the hybrid process (enzymatic saccharification after acidic hydrolysis). The yield of cellulosic ethanol conversion with those pretreatment processes were obtained as the following order : hybrid process > acidic hydrolysis > enzymatic saccharification. The optimum fermentation time was proven to be two days in this work. The yield of cellulosic ethanol conversion using celluclast after the acidic hydrolysis with 20 wt% sulfuric acid were obtained as the following order : sawdust > rice straw > copying paper > newspaper when conducting enzymatic saccharification.