Количественный анализ экспрессии опухолеассоциированных белков в образцах сывороток крови у больных раком яичника и у больных с другой локализацией опухоли. Возможности использования для диагностики и оценки распространенности опухолевого процесса

Abstract

The specific features of the expression of tumor-associated proteins (TAP) were immunologically studied in the sera of patients with ovarian cancer (OC) and other tumor location by means of immune sera (As) or monoclonal antibodies (MAb) to find out whether they could be used to diagnose and estimate the extent of a tumorous process. MAb 1 (to HEp-2 cell membrane proteins, larynx cancer), Ac4 (to a pool of two ovarian cystadenocarcinomas), and MAb 3 (to affinity-pu- rified proteins of the apparently intact human gastric mucosa) were used to examine the sera of patients with OC and other tumor location and positive responsiveness was detected in 82, ~100, and 77 % of cases, respectively. The differences in the expression of TAP in the patients versus healthy donors were shown to be statistically significant (p = 0.0001; p = 0.015; p = 0.01, respectively). The sensitivity of quantifying ELISA in detecting TAP was 78 and 85 % in patients with Stages I–II and III–IV OC, respectively; ~100 and 89 % in patients with breast cancer and in those with gastrointestinal tract cancer, respectively; and 60 and 14 % in patients with lymphopro- liferative diseases and healthy donors, respectively. Comparison of TAP detection rates in the authors’ test systems with multiplex testing with a biochip array of 12 tumor markers has shown that these test systems are at the world standard level.