カラム凝集法を用いた輸血検査における自動化の問題点 血しょう検体の有用性

Abstract

We introduced Auto-Vue (Ortho Co.), full automation system of column agglutination technology (CAT) to our range of routine tests in January 1999. Although it is advisable to use plasma samples when automation is a factor, it has been our practice to use serum in conventional tube technique (TT). We compared the titer of various antibodies of EDTA plasma with serum in the Auto-Vue examination and found no difference between them concerning sensitivity of detection. Next, we found that positive samples of self-controls were more numerous in the CAT method than in the TT. No instance of auto-antibody was detected in those samples whose self-controls were positive in CAT and negative in TT. Additionally, it was observed that higher globulin value, lower specific gravity of erythrocytes and higher leukocytes count were frequently associated with false positive results of self-controls in CAT. As a result, plasma samples were useful for detection of antibodies in the CAT method. These results allow for the standardization of transfusion tests, increasing the efficiency of tasks and returning the results of tests more rapidly than before by using the Auto-Vue system.