Загоєння ран шкіри щурів старечого віку за умов хронічної гіперглікемії та при застосуванні збагаченої тромбоцитами плазми

Abstract

Wound regeneration is a complex problem for modern healthcare, since the existing methods of treatment are not always effective, which necessitates the development of new promising methods. In this regard, today researchers are greatly interested in the possibility of using platelet-rich plasma in the treatment of patients with soft tissue injuries. The purpose of our research was to study the micro-, ultrastructural, planimetric and morphometric features of the skin reparative regeneration of old rats under the chronic hyperglycemia using platelet-rich plasma. Material and methods. The study was conducted on 30 white laboratory old rats: І group was a control group, ІІ group consisted of animals with chronic hyperglycemia of the body; ІІІ group included animals with chronic hyperglycemia of the body that were administered with platelet-rich plasma into the skin defect. The chronic hyperglycemia was simulated by the single intraperitoneal administration of streptozotocin (40 mg/kg) and nicotinic acid (1 mg/kg). The wound was formed by cutting out a piece of skin in the interscapular region. The animals with chronic hyperglycemia of the body ІІІ group were administered with autologous platelet-rich plasma into the skin defect. The studies were performed on the 14 days after trauma. The light microscopy was performed using an Olympus BH-2 microscope (Japan), ultramicroscopic study was performed using a JEM-1230 transmission electron microscope (JEOL, Japan), the planimetric analysis was performed using the morphometric program "Digimizer". The statistical analysis was performed using the SPSS-17 software package. Results and discussion. The obtained results showed that the process of skin regeneration in elderly rats with chronic hyperglycemia, which were injected with platelet-rich plasma, had a more favorable course than in animals that did not use the corrector. The wound area was reduced by 23.57 % (p = 0.654) than in rats with chronic hyperglycemia without correction and by 65.29 % (p = 0.857) larger than in the control group. It was shown that in animals with impaired carbohydrate metabolism, which received platelet-rich plasma, the beginning of the formation of the reticular and papillary layers dermis was observed. Conclusion. The vascular density of the microvasculature in animals with chronic hyperglycemia, which was injected with the corrector, was 22.89 % more (p = 0.246) than in animals simulated with chronic hyperglycemia without a corrector, but 15.69 % (p = 0.066) less than the control value