骨形成タンパク‐ヒドロキシアパタイト複合体による骨欠損部修復実験

Abstract

Utilization of bone morphogenetic protein (BMP) new bone production and regeneration after a bone defeet is urgently needed in clinical treatment. Sell curing hydroxyapatite (HAP) cement has a poss'bility to be a useful material as an effective BMP delivery system. In the present report, BMP-HAP compositions were implanted into femur tone defects of ICR mouse to examine the usefylness as an augumcntation materal and a delivery system with BNIP in the clinic.Materials and Methods:BMP was made by the method that was discovered by Urist in 1984. Crude BMP (c-BMP) was extracted from fresh bovine cortical bone.Five mg of freeze dried c-BMP and 20 mg of HAP cement were mixed and shape d as φ 3mm×5 mm cylindrical type of BMP-HAP composition. As the experimental group, the BMPHAP compositions were implanted into 5 mm length of regional bone and periostA urn defections which were splinted with Kirschner wire (K-wire) used for a space retainer on f femur bones of ICR mice. Tissue specimens were taken from animals at 1, 2, 3, 4, 6 weeks post-implantation to examine for histopathological evaluation. Only K-wires were put the same way as an experimental group for the sham control, and only HAP cement blocks were implanted into same region for comparisons with BMP-HAP composition.Results:1. In the wire Group, the bone defects between both bone edges of the femur bone were filled with connective tissue as a non-union at 4 weeks post-implantation.2. In HAP Group, the bony bridges were identified with a new bone from the surface of the existing bone were at 6 weeks post-implantation.3. In BMP-HAP composition group, the bone formations from the surface of existing bone to the implanted material were more active than the HAP group, but the bone inductions surrounding the implanted BMP-HAP composition were not so reactive.According to these results, I want to suggest that HAP cement is a u, eful matdrial as an atigumentation material and delivery system with BMP.