Abstract
ABSTRACT Objectives : WHW is a polyherbal medicine for the treatment of chronic rena l failure (CRF). WHW previously reported various biological property such as anti-inflammation, anti-oxidation and anti-renal fibrosis in CRF. This study aimed to investigate the anti-apoptotic effect of WH W on staurosporin(SSP)-induced apoptosis in canine kidney epithelial cells (MDCK).Methods : MDCK cells were treated with different concentrations of WHW (0.1, 0.2, 0.5 and 1 ㎎/㎖) for 1 h, and then induced apoptosis by treatment of SSP (1 μM) for 24 h. Cell viability was measured by WST-1 assay. The expression of apoptotic proteins such as caspase-3, Bax and Bcl-2 was determined by Western blot. Caspase-3 activity and ROS levels were also measured by their c ommercial available assay kits. Cell apoptosis was observed by Hoechst and DNA fragmentation. Results : WHW significantly increased the cell viability on SSP-treated MDCK cells. WHW inhibited SSP-induced expression of apoptotic proteins such as caspase-3 and Bax, and significantly decreased caspase-3 activity in MDCK cells. WHW significantly decreased SSP-induced production of ROS, and suppressed SSP-induced chromatin condensation and DNA fragmentation in MDC K cells.Conclusions : These results suggest that WHW has an anti-apoptotic effect in renal cells through suppressing the expression of apoptotic proteins, ROS production and DNA da mages.Key words : Anti-apoptosis, chronic renal failure, MDCK, polyherbal medicine, staurosporin, WHW
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