Abstract
Vital physiological and metabolic processes occurring in the human body are associated with free radical oxidation, which affects the structure, permeability and physico-chemical properties of biological membranes. Horse sorrel is used for scurvy, gout, diabetes, jaundice, hypertension, rheumatism, skin diseases – scabies, lichen, eczema, burns, as well as diseases of the digestive and cardiovascular systems. The purpose of the research: to study the technology of obtaining liquid extract of horse sorrel roots and to study its antioxidant activity on the body. Liquid extract of horse sorrel roots and auxiliary substances were used as the object of the study: dimethyl sulfoxide DMSO, ethyl alcohol 96%, quercetin, lecithin. When performing the work, equipment was used: liquid chromatograph "Color Yauza-01-AA", SF-LEKISS 1207UV. The object of the study of the total antioxidant activity were extracts of horse sorrel roots obtained using ethyl alcohol in concentration: 30%, 40%, 50%, 60%, 70%, 80%. The total content of antioxidants in the studied liquid extracts of horse sorrel roots was determined by two methods: using a liquid chromatograph "Color Yauza-01-AA" with an amperometric detector. The quantitative content of antioxidants was determined according to the calibration schedule. The conversion was carried out for gallic acid and quercetin. Using a model of iron-induced lipid peroxidation in the system of lecithin (phosphotidylcholine) liposomes, the studied liquid extracts were evaluated by reducing the accumulation of peroxide compounds. The study was carried out at a concentration of 200 mcg/ml to 500 mcg/ml of horse sorrel extract and increasing the dose.
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