Abstract
One hundred and seventeen isolates of Rhizoctonia solani Kuhn were obtained from creeping bentgrass [Agrostis stolonifera L. var. palustris (Huds.) Farw] and Kentucky bluegrass (Poa pratensis L.) with symptoms of brown patch disease on golf courses in Japan from 1997 through 2006. Based on colony morphology and restriction fragment length polymorphism (RFLP) analysis using the internal transcribed spacer region of ribosomal DNA (rDNA-ITS), 96 isolates (82.1%) were identified as R. solani AG-2-2 IIIB, and 21 isolates (18.0%) were R. solani AG-1 IB. On the basis of the results of this report, brown patch on creeping bentgrass is mainly caused by R. solani AG-2-2 IIIB nearly throughout Japan and on Kentucky bluegrass it is caused by R. solani AG-1 IB in the cool weather area in Japan.
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