Abstract

This work is aimed at investigating the composition and content of phenolic compounds contained in P. schlothauerae leaves using high-performance liquid chromatography (HPLC). The material for the study of flavonoids and phenolcarboxylic acids in extracts obtained from P. schlothauerae leaves was collected from natural P. schlothauerae populations during flowering and fruiting phases. Ethanol extracts obtained by extraction in a water bath with 40% ethyl alcohol were analysed using an Agilent 1200 liquid chromatograph equipped with a diode array detector. It is found that 40% water-ethanol leaf extracts contain chlorogenic, caffeic and n-coumaric phenolic carboxylic acids, as well as flavonols including aglycon quercetin, kaempferol and glycoside of quercetin hyperoside. Quercetin, kaempferol, caffeic and n-coumaric acids were found in the hydrolysates of water-ethanol extracts. Differences in the qualitative composition of phenolic compounds contained in the hydrolysates of water-ethanol extracts from P. schlothauerae leaves and plants of the Spiraea genus have been revealed, confirming the taxonomic independence of P. schlothauerae. The content of phenolic substances in P. schlothauerae leaves is higher during the fruiting phase. Kaempferol (0.07 mg / g) is found in water-ethanol extracts from leaves only during the fruiting phase.

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