Abstract
최근 울산광역시 소재의 넙치 양식장에서 체색 흑화, 간 위축, 장관 백탁 등의 증상을 보이며 넙치의 대량 폐사가 빈번히 발생하여, 병원체를 분리하고 감염 특성에 관하여 연구하였다. 2012년 5월 병어로부터 분리한 원인균은 생화학 시험과 16S rRNA, dnaJ gene을 이용한 염기서열 분석을 통해 V. scophthalmi로 동정하였다. 병원성 시험 결과, 본 시험 균주가 <TEX>$10^6$</TEX> CFU/fish에서 75%의 누적 폐사율을 보여 강한 병원성이 확인되었다. V. scophthalmi 감염어는 조직병리학적 병변으로서 간 위축, 장 상피 탈락, 장내 세포 물질 유출 및 장관백탁증 등이 확인되었다. Recently high mortality of cultured olive flounder, Paralichthys olivaceus occurred frequently at the fish farms in Ulsan, Korea. The diseased fish showed skinny body and swimming behavior around the water surface with liver atrophy and white enteritis as internal signs. The isolated bacteria were identified to V. scophthalmi by biochemical test, nucleotide analysis of 16S rRNA and dnaJ gene sequencing. The pathogen of this study showed strong pathogenicity as 75% mortality to olive flounder by intraperitoneal injection of <TEX>$1{\times}10^6$</TEX> CFU/fish. The pathological sign was not different between the naturally diseased fish and the artificially infected fish. Histopathological changes were shown to liver atrophy, desquamation of the intestinal mucosa and hyaline droplet like as other previous studies.
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