상백피에 의한 MC/9 비만세포의 활성 억제 조절 연구

Abstract

ABSTRACT Objective : Morus alba Linne Root Bark (MRAL) is a medicinal herb in Korean Medicine, known for its anti-inflammatory and anti-allergic properties. However, its me chanisms of action and the cellular targets have not yet been found and the study was developed to investigate the allergic suppressive effect of MRAL. The purpose of this study is to investigate the allergic suppressive effects of MRAL on activation of MC/9 mast cells.Methods : Cytotoxic activity of MRAL (50, 100, 200, 400 µg/mL) on MC/9 mast cells measured using EZ-Cytox cell viability assay kit (WST reagent). The levels of interleuk in-5 (IL-5), IL-13 and IL-4, IL-5, IL-6, IL-13 mRNA expression were measured by enzyme-linked immunosorbent as say (ELISA) and real-time PCR respectively. The expression of transcription factors such as G ATA-1, GATA-2, NFAT, AP-1 and NF-κB p65 DNA binding activity were measured by western blot and electrop horesis mobility shift assay (EMSA).Results : Our results indicated that MRAL (50 µg/mL, 100 µg/mL) significantly inhibited PMA/Ionomycin-induced production of IL-5 and IL-13 and the expr ession of IL-4, IL-5, IL-6 and IL-13 mRNA in MC/9 mast cells. Moreover, MRAL (50 µg/mL, 100 µg/mL) inhibited PMA/Ionomycin-induced GATA-1, GATA-2, NFAT-1, NFAT-2, c-Fos protein expression and NF-κB p65 DNA binding activity in MC/9 mast cells.Conclusions : In conclusion, we suspect the anti-allergenic activities of MRAL, may be related to the regulation of transcription factors GATA-1, GATA-2, NFAT-1, NFAT-2, c-Fos and NF-κB p65 DNA binding assay causing inhibition of Th2 cytokines IL-5 and IL-13 in mast cells.Key words : Morus alba Linne Root Bark (MRAL), MC/9 mast cell line, Anti-allergy, Transcription factor, Th2 cytokines