눈연꽃 추출물의 Lyn과 Syk 인산화 억제를 통한 알레르기성 염증 억제 효과

Abstract

Background: Snow lotus [Saussurea involucrata (Kar. & Kir.) Sch. Bip.] is used in Chinese herbal medicine because of its antioxidant and anti-inflammatory effects. The mechanism of its anti-inflammatory effect on allergic response is unknown.<BR>Methods and Results: We evaluated the effect of the aqueous extract of S. involucrata flowers on the allergic inflammatory response in dinitrophenyl-immunoglobulin E (DNP-IgE)-activated RBL-2H3 cells. Cell viability and β-hexosaminidase release from the activated RBL-2H3 cells were evaluated. The levels of proinflammatory molecules including tumor necrosis factor-alpha (TNF-α), interleukin (IL)-4, IL-10, IL-1β, and prostaglandin E2 in RBL-2H3 cells treated with 5% and 10% S. involucrata extract. Treatment with S. involucrata extract significantly suppressed the phosphorylation of protein kinase B (AKT), p38, c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK)1/2, and mitogen-activiated protein kinase (MEK) in a dose-dependent manner. S. involucrata extract also inhibited the Fc epsilon receptor 1 signaling pathway involving phosphorylation of Lyn, Syk, and phosphoinositide 3-kinase (PI3K), which is associated with the PI3K-AKT-ERK pathway and cytokine expression. A major mechanism of action of S. involucrata extract was to reduce the extent of the DNP-IgE-induced cytoskeletal change from globular to filamentous actin in RBL-2H3 cells.<BR>Conclusions: S. involucrata extract mediates its antiallergic effect by suppressing inflammation. The findings will be valuable in the development of S. involucrata extract as a phytomedicine with minimal side effects for allergic diseases.