СПЕКТРОФОТОМЕТРИЧЕСКОЕ ОПРЕДЕЛЕНИЕ L-ТИРОЗИНА В НАНОКОНСТРУКЦИЯХ НА ОСНОВЕ СУБЪЕДИНИЦ ВИРУСА ГРИППА B/FLORIDA/04/2006 И БИОРАЗЛАГАЕМОГО ПОЛИМЕРА ДЕКСТРАНА

Abstract

Our aim was to synthesize antigen polysaccharide complexes based on the surface subunits of the influenza virus B/Florida/04/2006 in the presence of various primary amines and to study their physicochemical properties. The antigen polysaccharide complexes were synthesized by conjugation with a partially oxidized dextran and subsequent reduction by natrium borane. The biological and physicochemical properties of the as-synthesized antigen polysaccharide nanoscale constructions are investigated. On the basis of the results, we have proposed a spectrophotometric method for determining tyrosine in antigen samples modified by oxidized dextran in the presence of tyrosine. Ratios between the main components of the synthesized antigen polysaccharide nanoscale constructions loaded with an additional amount of tyrosine have been determined. It is shown that the synthesized antigen polysaccharide complexes contain 16-30 parts of dextran and 40-130 parts of additionally taken amino acid residues per 1 part of antigens. Approximate molecular weights of the synthesized conjugates are shown to range from 1 to 2 million Da. Nanoscale constructions with such molecular weights are comparable with those of viral particles and, in fact, can be considered as virus-like particles.