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Abstract
Studying the effects of regulatory peptides on the stress-induced shifts in the bodily processes is of great fundamental and applied significance. Currently, a wide range of peptide neurotropic drugs, affecting the stress response development, are used in medicine, and new promising molecules are being studied. The study was aimed to assess the effects of the adrenocorticotropic hormone (ACTH) synthetic analog, ACTH(6-9)-Pro-Gly-Pro, administered at a dose of 5, 50 and 500 μg/kg, on the free-radical oxidation processes in Wistar rats, subjected to chronic restraint stress (CRS) during two weeks. Serum levels of 8-oxo-2'-deoxyguanosine (8-OHdG) and superoxide dismutase 3 (SOD3) were assessed by enzyme immunoassay, and the levels of thiobarbituric acid reactive substances (TBARS) were assessed by fluorimetric method. CRS lead to the significant increase in the 8-OHdG levels by 18.4% (p = 0.01) and the decrease in the SOD3 levels by 14.3% (p = 0.01), however, it had no effect on the levels of TBARS. ACTH(6-9)-Pro-Gly-Pro, administered at a dose of 5 and 50 μg/kg, significantly decreased the levels of 8-OHdG by 19.8% (p = 0.03) and 30% (p = 0.001), respectively. Thus, it was found that CRS resulted in oxidative stress in animals. ACTH(6-9)-Pro-Gly-Pro administration at a dose of 5 and 50 μg/kg inhibits the stress-induced free-radical oxidation processes.
Highlights
Studying the effects of regulatory peptides on the stress-induced shifts in the bodily processes is of great fundamental and applied significance
The rats were divided into five groups, 11 rats per group: 1 — intact animals (administration of normal saline (NS) with no stress applied), 2 — control group (CRS + NS), 3 — chronic restraint stress (CRS) + adrenocorticotropic hormone (ACTH)(6-9)Pro-Gly-Pro 5 μg/kg, 4 — CRS + ACTH(6-9)-Pro-Gly-Pro 50 μg/kg, 5 — CRS + ACTH(6-9)-Pro-Gly-Pro 500 μg/kg
The study found that CRS resulted in oxidative stress (Fig. 2)
Summary
Studying the effects of regulatory peptides on the stress-induced shifts in the bodily processes is of great fundamental and applied significance. The study was aimed to assess the effects of the adrenocorticotropic hormone (ACTH) synthetic analog, ACTH(6-9)-Pro-Gly-Pro, administered at a dose of 5, 50 and 500 μg/kg, on the free-radical oxidation processes in Wistar rats, subjected to chronic restraint stress (CRS) during two weeks. ACTH(6-9)-Pro-Gly-Pro, administered at a dose of 5 and 50 μg/kg, significantly decreased the levels of 8-OHdG by 19.8% (p = 0.03) and 30% (p = 0.001), respectively. ACTH(6-9)-Pro-Gly-Pro administration at a dose of 5 and 50 μg/kg inhibits the stress-induced free-radical oxidation processes. Введение АКТГ(6-9)-Pro-Gly-Pro в дозах 5 и 50 мкг/кг оказывает ингибирующее влияние на стресс-индуцированные процессы свободнорадикального окисления. И. Бобынцев — концепция и дизайн исследования, написание рукописи; О.
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