Abstract

한방제제 Herb <TEX>$Mix^{(R)}$</TEX>(Herb BIO Co.)에 당귀, 작약, 천궁을 강화한 개량 한방제제(Herb Mix <TEX>$Gold^{(R)}$</TEX>)의 상대적 효능과 적정 첨가 수준을 결정하기 위해 사양 시험을 실시하였다. 시험은 45주령 산란계(Hy-Line Brown) 720수를 대조구 포함하여 총 6개 처리구로 처리당 6반복 반복당 10케이지, 케이지당 2수씩 수용하여 난괴법으로 배치한 후 5주간의 사양 시험을 실시하였다. 처리구는 대조구(T1), Herb <TEX>$Mix^{(R)}$</TEX> 0.2% 첨가구(T2), Herb Mix <TEX>$Gold^{(R)}$</TEX> 0.1% 첨가구(T3), Herb Mix <TEX>$Gold^{(R)}$</TEX> 0.2% 첨가구(T4), Herb Mix <TEX>$Gold^{(R)}$</TEX> 0.3% 첨가구(T5) 그리고 항생제 <TEX>$Avilamycin^{(R)}$</TEX> 2%를 함유한 <TEX>$Avilamix^{(R)}$</TEX> (CTC BIO Co., Ltd)를 0.03% 사용하여 6 ppm 첨가구(T6)를 두었다. 실험 결과 산란율은 Herb Mix <TEX>$Gold^{(R)}$</TEX> 0.2% 첨가구가 가장 높았으며(P<0.05) 다음으로 Herb Mix <TEX>$Gold^{(R)}$</TEX> 0.3% 첨가구, Herb <TEX>$Mix^{(R)}$</TEX> 0.2% 첨가구, Herb Mix <TEX>$Gold^{(R)}$</TEX> 0.1% 첨가구, Avilamycin첨가구 그리고 대조구 순이었다. 산란 지수도 산란율과 같은 경향이었으나 통계적 유의차는 없었다(P>0.05). 난중, 연파란율, 사료 섭취량 및 사료 요구율과 계란의 품질 평가 항목인 난각 강도, 난각 두께, 난각 색깔, Haugh unit 및 난황 색깔에서 처리간에 유의한 차이는 없었다(P>0.05). 장내용물 중 Cl. perfringens와 E. coli의 수는 첨가구들이 대조구에 비해 적었고 Lactobacilli의 수는 Herb Mix <TEX>$Gold^{(R)}$</TEX> 첨가구들이 높은 경항을 보여주었다(P>0.05). 백혈구 (WBC) 수와 heterophil(HE)의 경우 첨가구들이 대조구에 비해 적었으며 림프구 (LY)의 경우 모든 첨가구들이 높은 경향을 보였다(P>0.05). Stress indicator로 알려진 HE/LY는 Herb Mix <TEX>$Gold^{(R)}$</TEX> 0.2% 첨가구에서 낮은 경향을 나타냈다(P>0.05). 적혈구나 헤모글로빈은 모든 첨가구들이 대조구에 비해 증가하는 경향을 보였으며 특히 Herb Mix <TEX>$Gold^{(R)}$</TEX> 0.2%구가 높은 경향을 나타내었다(P>0.05). 결론적으로 산란 생산성 개전을 위해 Herb Mix <TEX>$Gold^{(R)}$</TEX> 0.2% 첨가가 적당하다고 사료되며, 이는 선발 원료로 강화된 한방제제가 장내 미생물 균총, 혈액 내 백혈구 및 적혈구 수에 유익하게 미치는 영향에 기인한 것으로 사료된다. This study was conducted to investigate the effects of graded levels of a herbal recipe(Herb Mix <TEX>$Gold^{(R)}$</TEX>, Herb BIO Co.) supplemented to a commercial layer diet. The Herb Mix <TEX>$Gold^{(R)}$</TEX> is an improved recipe of Herb <TEX>$Mix^{(R)}$</TEX>, fortified with Angelica gigas, Discorea japonica and Ligusticum jeholense. A total of 720 layers (Hy-Line Brown) of 45 wks old were assigned to one of six treatments; control, 0.2% Herb <TEX>$Mix^{(R)}$</TEX>, 0.1%, 0.2%, and 0.3% Herb Mix <TEX>$Gold^{(R)}$</TEX>, and 6 ppm Avilamycin. Each treatment had 6 replicates of 20 birds each housed in 2 birds cages. Birds were fed diets and water ad libitum for 5 weeks. Hen-day egg production was significantly (P<0.05) different among treatments. Herb Mix <TEX>$Gold^{(R)}$</TEX> 0.2% treatment showed the highest egg production followed by Herb Mix <TEX>$Gold^{(R)}$</TEX> 0.3%, Herb <TEX>$Mix^{(R)}$</TEX> 0.2%, Herb Mix <TEX>$Gold^{(R)}$</TEX> 0.1%, Avilamycin 6 ppm and the control. Hen-housed egg production, egg weight, soft and broken egg ration, feed intake, feed conversion ratio, shell strength, shell thickness, shell color index, Haugh unit and yolk color index were not significantly different among treatments. Nor was cfu of Cl. perfringens and E. coli and Lactobacilli in the small intestinal content significantly different among treatments. The number of white and red blood cells, hemoglobin, heterophil, lymphocyte, thus heterophil to lymphocyte ratio were not significantly modified. It was concluded that Herb Mix <TEX>$Gold^{(R)}$</TEX> at the level of 0.2% in the layer diet improves laying performance.

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