캄보디아 프놈보콜국립공원의 Balanophora fungosa var. indica의 숙주식물에 대한 DNA barcoding 기법을 통한 동정

Abstract

ABSTRACT: During the floristic survey on Phnom Bokor National Park, Kampot, Cambodia, we encountered Balanophorafungosa var. indica, which is a tropical holoparasitic plant. To identify its host species, we collected host roots and trees nearbyand tried to identify them using DNA barcoding approach. We applied plastid rbcL and matK gene regions as DNA barcodemarkers, and successfully amplified and sequenced the markers from 15 host roots and seven tree samples. Obtained host rootsequences were identified as Primulaceae, Celastraceae, Myrtaceae, and Oleaceae, while trees nearby are Oleaceae, Myrta-ceae, Sapindaceae, Rosaceae, Clusiaceae, Ericaceae, and Lauraceae. At genus level, host species are identified as Myrsine,Euonymus , Syzygium, and Olea, but failed in species discrimination. Myrsine (Primulaceae) and Olea (Oleaceae) are reportedhere as host species of B. fungosa var. indica for the first time. Further sampling and comparative work, and DNA barcodingwill help recognize the biodiversity of the area and host species of Balanophora, together with their evolution.