海〓の日本腦炎感染に関する実験的研究 (第5報)

Abstract

In the brain tissues of the guinea-pigs, immunized against the Japanese B encephalitis with the intracerebral inoculation of the active or inactivated virus, the intracerebral concentration of the neutralizing antibody can be observed in loco. Namely, certain period of time after the intracerebral or subcutaneous antigen inoculation, the brains of all the animals were perfused with saline and the neutralizing antibody contained in the perfused brain tissues, excluding the participation of the serum antibody of the circulating blood in the brain, was titrated, and it was ascertained that the perfused brain tissues of the intracerebrally immunized animals contained the antibody in higher titer than those of the subcutaneously immunized ones, even when the serum antibody is present in nearly the equivalent titer in the circulating blood of the animals in both the intracerebral and subcutaneous immunization groups. In regard to this fact, the following two phenomena may be able to be considered as the substantial cause of it, namely, in the first place, the local intracerebral antibody production in situ, and secondly, the permeation into and the adherence to the brain tissue of the serum antibody of the circulating blood. In the present study it was elucidated that the titer of the antibody in the brain tissue rises significantly either later than or at least concomitantly with and not at all earlier than that in the serum of the circulating blood. It was also made clear, by the antibody titration on the perfused brain tissues, that after the repeated intracerebral immunization the antibody in the brain tissue rises and falls utterly in accompaniment with that in the circulating blood, and also that the former is present as long as the latter is present and disappears if the latter disappears. Standing upon the results described above, it might be possible to consider that the substantial causes of the intracerebral antibody concentration is principally the permeation and adherence of the serum antibody to the brain tissue, owing to the rise of permeability of the blood-brain-barrier caused by the brain injury such as the intracerebral antigen inoculation.