Abstract
In order to examine the feasibility of adoptive immunotherapy by IL-2 activated killer cells (LAK cells) and rIL-2 in oral cancer patients, the induction of LAK cells in serum-free medium instead of human serum contained medium was studied.The following results were obtained.1. The LAK cells induced in serum-free medium (LAK-SF cells) had as high cytotoxic activity against Raji and K562 cells as the LAK cells in human serum contained medium (LAK-S cells)2. As the result of flow cytometric analysis, both LAK-SF and LAK-S cells expressed similar cell surface markers.3. It was shown that transferrin was a significant factor and insulin worked as a suppressive factor to induce the LAK cells in serum-free medium.4. It was revealed that cytotoxic activity of LAK-SF cells was higher than that of LAK-S cells against head and neck carcinoma cell lines.As a result of the foregoing studies, the LAK cells induced in serum-free medium could be applied to adoptive immunotherapy in oral cancer patients.
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