Abstract
We established a cell line (Sa3) from human oral cancer. It was derived from a biopsy specimen of a squamous cell carcinoma in the upper gingiva of a Japanese man. The cell culture was initiated by explant culture, and the cell line has now been growing in vitro for more than 80 passages.The cells had an epitheloid morphology and grew as adherent monolayers. They had a doubling time of 55 hours and colony formation of 34%. Chromosomal numbers ranged from 36 to 76 and the mode was 71. The cell line was tumorigenic in nude mice and histologically identifiable with the original tumor. The cells were sensitive to hyperthermia.Histochemical staining of the cells for lysosomal enzymes and cytokeratin was positive.
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