Abstract
Protocorm-like body (PLB) of Cymbidium finlaysonianum Lindl. (epiphytic species) and rhizome of Cymbidium kanran (terrestrial species) were cultured on modified Murashige and Skoog (MS) medium supplemented with various concentrations of sucrose and trehalose. Shoot differentiation in PLB cultures of C. finlaysonianum are enhanced by the addition of trehalose at concentrations of 10g/l and 20g/l. Sucrose at concentrations of 10g/l and 20g/ l had lesser effects on shoot formation from PLB cultures. Root formation from shoots developed from PLBs was significantly promoted at treatment of 5g/l trehalose. Shoot differentiation from rhizome cultures of C. kanran was significantly promoted by the addition of trehalose at concentrations of 10, 20 and 40g/l. Root formation from shoot developed from rhizome occurred in the presence of trehalose at concentrations 5, 10 and 20g/l. Sucrose had no effect on root formation from shoots developed from rhizome cultures.
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