Abstract
Subsequent to the previous work, casein hydrolyzates obtained by treatment with pepsin, trypsin or the lyophilized hepatopancreas of prawn were compared with intact casein as a nitrogen source of diet for the prawn, Penaeus japonicus, by a feeding experiment. Casamino acids (DIFCO) and casitone (DIFCO) were also tested. The level of nitrogen source was 52.5% in all test diets. In a 4-week feeding trial, all test diets containing casein hydrolyzates resulted in poor growth and high mortality. These results suggest that peptides as well as free amino acids are far inferior to protein as a nitrogen source in the prawn diet.
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