Abstract
The effects of blood stirring on various plasma constituent levels of blood samples collected from the caudal vessels of anesthetized (MS-222, 100mg/l) carp, Cyprinus carpio (body weight: about 500g) with a plastic syringe tipped with a 20-gauge stainless steel needle were investigated.Ten or a hundred time-stirring of blood samples by manual inverting at 60 times per minute did not affect the plasma levels of total protein, albumin (by BCG method), glucose, urea-N, total cholesterol, inorganic phosphate, magnesium, GOT, GPT, LDH, LAP, CK, Al-P and Acid P. But Acid P activity was increased or decreased by 300 time-stirring.With the blood smear stained with the Leishman-Giemsa stain, effect of 5 time-stirring on morphological change of thrombocytes and leucocytes was not recognizable. But their deformity or karyolysis were observed frequently in case of 50 time-stirring.The contamination of plasma with thrombocytes or leucocytes increased plasma levels of GOT, GPT, LDH, Acid P, CK, calcium, magnesium, inorganic phosphate, especially Acid P activity.The inorganic phosphate level of plasma collected by a plastic syringe was significantly higher than that by a glass syringe. GOT and Acid P activities changed indifferently to the quality of syringe.Light blood stirring does not affect the plasma constituent levels, but caution must be taken in selecting the material of blood sampling appliances.
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