リポ蛋白リパーゼ (肝性リパーゼ) の血小板機能に及ぼす影響について

Abstract

The influence of hepatic triglyceride lipase isolated from human post-heparin plasma by the modified method of Ehnholm et al on the platelet aggregation was studied. 0.5ml of the platelet rich plasma (PRP) or the washed platelet rich suspension (WPRP) obtained by the albumin density gradient separation method of Walsh was incubated with 0.5ml of hepatic triglyceride lipase (HTGL) or the buffer as a control for 5 minutes at 37°C, or additionally for 55 minutes at 28°C (in total 60 minutes). The platelet aggregation was induced by collagen, ADP or thrombin after the incubation.Results were (1) The reduction of the platelet aggregation in the HTGL group was observed in PRP. (2) The greater reduction of the collagen-induced aggregation was seen after the longer incubation for 60 minutes with HTGL. The difference between the HTGL and the control groups was significant. (3) The platelet aggregation in WPRP inclined to be reduced after the incubation with HTGL, but not so remarkable as in PRP. (4) The contamination of antithrombin III in isolated HTGL was found, but did not influence on the platelet aggregation. (5) The reduction of the platelet aggregation by HTGL was similar to that in the post-heparin PRP.We concluded from these results that HTGL inhibited the platelet aggregation, probably because of the changes in the lipid and prostaglandin metabolism in platelets, on which the changes in lipid metabolism in the plasma reflected partially.