Abstract
The surface structure of a model membrane composed of a Millipore filter and a synthetic lipid analogue, i.e., dioleylphosphate (DOPH), immersed in KC1 solution changes when CaCl2 concentration reaches a critical value. By the stereoscopic microscope mounted a photomultiplier on the eyepiece the structure change can be measured quantitatively as an intensity change of reflected light of the membrane surface. Calcium ions decreased finally the intensity to more or less 50% as compared with the original hydrophilic state whose solution was only KC1. The structure changes were observed with various adsorbed densities of DOPH, solution temperatures and pH. The degree of structure change was dependent on these conditions.The structure observation can also be used to detect the CaCl2 concentration in KCl solution. Since the critical CaCl2 concentration shifts widely with various densities of DOPH adsorbed in Millipore filter, the detection range was from 0.1 to 50mM CaCl2.
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