イカ甲骨の抗しゅよう性物質 Ｉ 試料の調製と抗しゅよう活性

Abstract

The internal shell of squid was extracted with hot M/100 EDTA after washings with acetone and hot water. The crude extract was obtained by dialysing the EDTA extract against water followed by freeze-drying. The crude extract exhibited antitumor activity for mouse sarcoma-180 (ascites type). Upon intraperitoneal injection of 10mg/kg/day×5 and 50mg/kg/day×5 of this material, the growth of mouse ascites carcinoma was inhibited by 43.7 per cent and 55.1 per cent respectively. The homogeneity of the crude extract was determined by column chromato-graphy on CPG10-170 and Sephadex G-200. The data suggested that the polysaccharide was separated from the protein and that both the polysaccharide and the protein were heterogenous. Chemical analysis showed that the crude extract contained 6.2 per cent N, 17.2 per cent sugar, 45.3 per cent protein, and 20.5 per cent ash.