Abstract

In order to measure the serum levels of IgG and IgG subclass antibodies against Japanese cedar pollen, an ELISA procedure was developed with commercially available antigen-coated wells for measuring IgE antibodies. The specificity, sensitivity and reproducibility of this ELISA system were all highly reliable in our experiments. The results of this study suggest the clinical usefulness of this method for following the responses of serum IgG and IgG subclass antibodies during immunotherapy for Japanese cedar pollinosis.

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