移植・再植歯の保存方法に関する研究 保存条件の相違による歯根膜細胞の生物学的活性について

Abstract

There is an intimated relation between the condition of periodontal ligament tissue and the success of tooth transplantation and replantation. In this study, the biological activity of periodontal ligament tissue after tooth extraction and storage was investigated to establish a long-term storage method for periodontal ligament tissue.Rat incisors were used as the study teeth. UW solution, Euro-Collins solution, DMEM, and sterile physiological saline were used as storage solution. The storage condition were refrigeration (4°C), controlled-freezing point storage (CFP storage;-2°C), and frozen (-80°C), and the storage period was 1-28 days. At the end of the storage period, cell cultures and double fluorescent staining were performed.The results of the cell cultures showed better growth of the periodontal ligament cells in the CFP-storage group than in the refrigeration-storage group or the frozen-storage group, and the results for the 4 different types of storage solution showed the best growth in the UW solution storage group, with a maximum storage period of 21 days. Similarly, double fluorescence staining showed the greatest viable cell fluorescence level in the CFP-storage and UW solution group.The above findings suggested that CFP storage in UW solution is useful for long-term storage of periodontal ligament tissue.