Abstract
The biologically active compounds (chlorogenic acid, caffeic acid, chicoric acid and 3, 5-dicaffeoylquinic acid) in lettuce (Lactuca sativa L.) were determined by high performance liquid chromatography (HPLC) to evaluate the difference between ten kinds of cultivars cultured with hydroponic solution at two levels of electric conductivities (EC ; 2.0 and 3.5 mS/cm). All the HPLC profiles of the cultivars tested showed the peaks at the same retention time ; there being no qualitative difference with the EC level. However, the peak areas differed with the cultivar. Particularly, a cultivar 'Chimasanchi' was found to contain much larger amounts of chlorogenic acid and 3, 5-dicaffeoylquinic acid than the others. The main component of 'Chimasanchi' was chlorogenic acid, while that of the others was chicoric acid. In addition, higher concentrations were obtained in the cultivars cultured with 3. 5 mS/ cm than with 2.0 mS/cm. Therefore, the concentration of secondary metabolites having biologically activities in vegetables may vary with the cultivars and also with the EC level of the culture solution, indicating that they could not be controlled by the chemical breeding and/or the culture condition.
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