Abstract
For the purpose of studying urinary proteins electrophoretically, a highly sensitive silver staining method was applied to urinary protein fractions separated by SDS-polyacrylamide gel electrophoresis. With this new method, urine specimens from normal human subjects, containing about 10mg/dl protein, could be separated into 26 protein bands without any pretreatment. Proteins having molecular weights from 230, 000 to 15, 000 were included in these bands. Three protein bands with molecular weights of 94, 000, 64, 000 and 15, 000 were found to be present in all of the 27 urine specimens from normal human subjects, while two protein bands with molecular weights of 82, 000 and 47, 000 were detected in 22 cases (80%). The protein bands with molecular weights of 94, 000, 64, 000 and 15, 000 were identified by immunoblotting technique to be Tamm mucoprotein, albumin and, β2-microglobulin, respectively.
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