Abstract
Salivary proteins contributing to the maintenance and stabilization of the denture base were analysed by means of the isoelectric focusing in agarose. Since this electrophoretic method has little been applied to the analysis of salivary proteins, we have carried out a basic study on isoelectric focusing of the mixed saliva, parotid saliva and serum samples collected from apparently healthy people.As a result of comparison between Saravis' method and the method of L. K. B. Co., the latter electrophoretic condition was found to present more consistent electrophoretic patterns. The cathodal side was found to be better than the anodal side for sample application. The pH gradient in the agarose gel after the electrophoresis was also satistactory. Clearer staining was obtained by the use of 0.25% Coomassie Brilliant Blue R-250 than by 0.25% Coomassie Brilliant Blue G-250.The mixed saliva was separated to 20 protein bands, the parotid saliva was to 14 bands, and the serum was to 21 bands. The main bands for the mixed saliva were those at 7.3 and 4.9, those for the parotid saliva were at pI=7.3 and 6.8, and those for the serum were at pI=6-7 and 4.9
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