炎症反応による免疫応答の増幅 ＩＬ‐１との関連において

Abstract

Inflammation and immune response are two major host defence mechanisms. It seems plausible that preceded inflammatory response may regulate the following immune response. We have studied this possibility using the casein-induced peritoneal inflammation. When antigen (sheep red blood cells, SRBC) were introduced into the peritoneal cavity of mice 3-9 hr after intraperitoneal injection of casein, enhancement of IgM responce in spleen was observed 4 days later. This enhancement was also observed in vitro primary antibody responce using spleen cells and SRBC when early inflammatory peritoneal exuded cells (PEC) were added into the culture but not observed by adding the later PEG. These early PEG were composed mostly of polymorphonaclear leukocytes (PMN), and the purified PMN in early PEC were found to contain an immune enhancing factor. The blood PMN which are origin of exuded PMN, could be activated to synthesize the factor (PMN factor) . This factor acts mainly on T cells and makes them produce lymphokines such as interleukin-2 and also has a pyrogenie activity. Thus this factor resembles interleukin-1 (IL-1) in terms of its biologic activity. Then, the possibility wheter the factor also resembles IL-1 in molecular structure was studied using purified PMN factor from rabbit. The factor had mw. 18500 and pl 7.2, and N terminal amino acid sequence was determined and highly homology with human IL-1 β was observed. Then PMN factor is considered to be a member of IL-1 family. Structure and biologic fenction of IL-1 was also reviewed.