濃厚血小板（ＰＣ）の迅速調製法

Abstract

The use of the citrate-phosphate-dextrose solution (the CPD solution) in place of the acidified citrate-dextrose solution (the ACD solution), as an anticoagulant solution has led to the slow resuspension of packed platelet sediments. Then, we studied for the state of resuspension of packed platelet sediments using the platelet concentrate (PC) prepared with supplement of the CPD solution, and obtained good results. That is, the rapid resuspension of packed platelet sediments was observed by adopting this new method. The platelet yield on the preparation of PC according to the present method was at least better than that of PC prepared by the standard procedure. In the PC obtained by the proposed method, the platelet functions were kept satisfactory; for example, both the aggregation, using collagen as a aggregating agent, and the percent recovery from hypotonic shock (%HSR) of 48-h stored platelet were remained about 80% of the initial activities. And a special triple bag system containing 26ml of the CPD solution in the primary bag and 10.6ml of the CPD solution in one of the two satellite bags, allows preparation of the PC under the closed condition.