Abstract
Recent studies have shown that residual leukocytes, especially lymphocytes in the huffy coat-poor red cell concentrate (BPRCC) prepared by the “top and bottom system” were significantly lower in number than those prepared by the conventional system. In this study we investigated the effects of different anti-coagulants on the composition of blood components prepared by the “top and bottom system”.We compared the characterization of whole blood collected in a citrate-phosphate-dextrose solution (CPD-WB) to that collected in an acid-citrate-dextrose solution (ACD-WB). The mean corpuscular volume of red cells in CPD-WB was smaller than that in ACD-WB. Although the same number of red cells existed in whole blood, the hematocrit (%) in CPD-WB was significantly lower than that in ACD-WB. After centrifugation, the red cells in CPD-WB were more concentrated than those in ACD-WB. The condition of concentrated red cells in the different anti-coagulants was also observed by freeze-replica electron microscopy. The recovery rate of plasma were 88.1±1.7% (n=25) and 83.8±2.1% (n=36) in platelet poor plasma prepared from the CPD-WB and the ACD-WB, respectively.The removal rates of leukocytes in BPRCC prepared from CPD-WB and ACD-WB were 78.4±10.4% (n=25), 70.4±8.9% (n=36), respectively. We conclude that the CPD-WB is more suitable than ACD-WB to prepare BPRCC with a high removal rate of leukocytes and high recovery of plasma when the BPRCC is prepared by the “top and bottom system”.
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