Abstract
A special feature of Y. pseudotuberculosis strains is its biochemical uniformity irrespective of the time and location of the causative agent isolation and the existence of 21 serological variants. Y. enterocolitica is a quite a heterogeneous species and is classified into 6 biochemical types associated with 29 serological variants. 221 Y. pseudotuberculosis and 447 Y. enterocolitica strains in total isolated in Siberia and in the Far East were characterized. Y. pseudotuberculosis genotype dominating in the Siberian and Far Eastern regions is presented by О:1b serotype of the first genogroup (pYV+, ympA+, HPI-) in two- (47:82 МDа) or single-plasmid (47 МDа) variants. Ribotyping and fingerprinting revealed 8 and 10 Y. pseudotuberculosis genotypes, respectively, that indicated relative heterogeneity of the circulated strains. Regional difference of ribotypes and fingerprints was noted. 401 of 447 Y. enterocolitica strains were classified as biotype A1 including 11 serotypes (O:4,32; O:4,44; O:5; O:6,30; O:6,31; O;7,8; O:12,25; O:13,7; O:19,8; O:41,43) and 46 strains belonged to biotypes 2–4 of O:3 and O:9 serotypes. Y. enterocolitica strains of biotypes 1A were isolated both from the environments, animals and patient samples as like the representatives of biotypes 2–4. The differentiating tests of fucose and sorbose made it possible to identify two species new for the Russian Federation – Y. mollaretii and Y. bercovierii. Y. enterocolitica biotypes 2–4 carried pYV plasmid and chromosomal ail, ystA virulence genes. These strains were referred to phagotypes Х3 (2/O:9) and VIII (4/O:3) and also to phagotype Xz (3/O:3), unique for Russia. Y. enterocolitica biotype 1A containing ystB thermostable toxin gene was confirmed to be an infectious etiological agent.
Highlights
Доминирующий в регионе генотип Y. pseudotuberculosis представлен О:1b серотипом первой геногруппы, содержит плазмиды 47:82 МDа или только 47 МDа, отличается спектром риботипов и фингерпринтов
Y. enterocolitica is a quite a heterogeneous species and is classified into 6 biochemical types associated with 29 serological variants
221 Y. pseudotuberculosis and 447 Y. enterocolitica strains in total isolated in Siberia and in the Far East were characterized
Summary
Всего были изучены 221 штамм Y. pseudotuberculosis и 447 штаммов Y. enterocolitica. Все манипуляции с ДНК Y. pseudotuberculosis и Y. enterocolitica для определения генов суперантигена YPM, ail, ystA, ystB, островов патогенности HPI и YAPI, инвазивности, плазмиды вирулентности pYV (выделение, структура праймеров, программы амплификации, состав реакционной смеси) были аналогичны, использованным в работе H. Все штаммы изучали на наличие плазмид: pVM 82MDa и pYV 47MDa у Y. pseudotuberculosis, pYV 47MDa в Y. enterocolitica, – по методу T. У 154 штаммов Y. pseudotuberculosis был определен риботип (Eco R1и Eco RV) и фингерпринт (IS 285и IS 1541) в лаборатории зоонозных и бактериальных инфекций НИИЭМ им. У 47 штаммов Y. enterocolitica проведено фаготипирование по методу Грация в лаборатории иерсиниозов (руководитель лаборатории – профессор Элизабет Карниэль) института им. СанктПетербург), для Y. pseudotuberculosis было проведено серогенотипирование [3]
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