소시지와 야채 샐러드에서 Yersinia enterocolitica 검출을 위한 배지법과 real-time PCR법의 비교

Abstract

Division of Food Chemical Residues, National Institute of Food and Drug Safety EvaluationAbstract The purpose of this study was to compare a conventional culture method and real-time PCR for the detectionof Yersinia enterocolitica (Y. enterocolitica) in sausage and in vegetable salad. Food samples inoculated with Y.enterocolitica were enriched in peptone-sorbitol bile-broth, and swabs were then streaked onto cefsulodin-irgasan-novobiocin agar. Biochemical tests for suspected colonies were performed with an API 20E strip. In parallel, real-timePCR was performed, targeting the 16S rRNA gene using 1 mL of enrichment broth. In sausage, the number of positivesamples detected by culture method (49 out of 60) was similar (p>0.05) with that of real-time PCR (50 out of 60).However, the number of positive samples of real-time PCR (26 out of 60) was significantly higher (p<0.05) than that ofthe conventional culture method (6 out of 60) in vegetable salad. Real-time PCR could be an effective screening tool fordetecting Y. enterocolitica, particularly in food samples with high levels of background flora, such as a vegetable salad.Keyworlds: Yersinia enterocolitica, culture method, real-time PCR, food