Abstract
Bacteria of the species V. parahaemolyticus are halophilic microorganisms that may be causing acute enteric disease in humans, occurring according to the type of foodborne illness. The success of the treatment-and-prophylactic and sanitary-epidemiological measures aimed at the prevention of the proliferation of «halophiles» largely depends on timely and accurate identification of strains of Vibrio secreted from patients, seafood, as well as in monitoring of environmental objects. This paper presents a method of identifying strains of the species V. parahaemolyticus by PCR in «real time» (Real-Time PCR), which enables the selection of chromosomal DNA from the test material, the PCR with the designed by us primers specific to the portion of a gene collagenase vppC and the probe, allowing to detect amplificatory the fragment and to incorporate the results in real-time.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: ЗДОРОВЬЕ НАСЕЛЕНИЯ И СРЕДА ОБИТАНИЯ - ЗНиСО / PUBLIC HEALTH AND LIFE ENVIRONMENT
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
