흰깨 추출물과 β-Sitosterol이 H1299 폐암세포의 성장, 이동, 부착에 미치는 효과

Abstract

The current study aimed to investigate effects of ethanol extract of white sesame seed (WSE) as well as a major constituent of white sesame seed, β-sitosterol, on the growth, migration, and adhesion of H1299 human lung cancer cells. Treatment with WSE at concentrations of 150, 300, and 600 μg/mL dose-dependently inhibited cell growth (to 51.5∼82.6% of control). Treatment with β-sitosterol at concentrations of 3.125, 6.25, 12.5, and 25 μM inhibited cell growth to a greater extent (to 27.5∼49.0% of control) than that with WSE (P<0.05). Treatment with WSE (at concentration of 600 μg/mL) or β-sitosterol (at concentration of 25 μM) resulted in increased sub-G1 cell population, indicating their apoptosis-inducing activities. β-Sitosterol was effective in inhibiting both cell migration (to 80.8∼86.2% of control at a concentration range of 3.125∼25 μM) and adhesion (to 21.5∼37.4% of control at a concentration range of 6.25∼25 μM), whereas WSE at a concentration range of 150∼600 μg/mL was ineffective. These results indicate that β-sitosterol is more active than WSE in inhibiting growth, migration, and adhesion of H1299 human lung cancer cells. Further studies are needed to determine if similar effects are reproduced in vivo.