Abstract
전기장의 세기 20 kV/cm, 펄스 폭 25 <TEX>${\mu}s$</TEX>에서 펄스 빈도 35 Hz(40 kJ/L), 55 Hz(70 kJ/L), 72 Hz(100 kJ/L) 및 85 Hz(130 kJ/L)로 달리하여 투입 에너지를 기준으로 사과 착즙액의 PEF 연속처리 살균 효과를 분석한 결과 일반세균수은 초기 5.3 log CFU/mL에서 130 kJ/L의 에너지로 처리하였을 때 3.0 log CFU/mL로 감소하였고 효모 및 곰팡이는 초기 4.6 log CFU/mL에서 130 kJ/L의 에너지로 처리로 < <TEX>$10^1$</TEX> CFU/mL 이하로 감소하였다. 그리고 대장균군수도 초기 4.7 log CFU/mL에서 130 kJ/L의 에너지 처리로 < <TEX>$10^1$</TEX> CFU/mL 이하로 감소하였다. PEF 처리에 따른 가용성 고형분, 유리당 등에는 처리 조건에 따른 유의적 차이가 없었으며(p<0.05), 총 페놀함량, DDPH 및 FRAP 값은 처리조건에 따라 부분적으로 유의적인 감소를 보였지만 <TEX>$65^{\circ}C$</TEX>에서의 가열처리보다는 감소량이 작았다. 전기장 세기 20.0 kV/cm, 펄스 빈도 85 Hz, 펄스 폭 25 <TEX>${\mu}s$</TEX>에서의 반복처리는 미생물 살균 효과가 제한적이었고 처리회수에 따라 총 페놀함량, DPPH 및 FRAP 값을 유의적(p<0.05)으로 감소시켰다. Apple juices were sterilized by continuous pulsed electric field (PEF) treatments of pulse width of 25 <TEX>${\mu}s$</TEX> at electric field intensity of 20.0 kV/cm, and with the varied pulse frequencies of 35 Hz (40 kJ/L), 55 Hz (70 kJ/L), 72 Hz (100 kJ/L) and 85 Hz (130 kJ/L). The PEF treatments of apple juice reduced the microbial counts from 5.3 log CFU/mL of initial state to 3.0 log CFU/mL after PEF treatment at energy density of 130 kJ/L. Also yeast and fungi after PEF treatments were reduced from 5.3 log CFU/mL to 3.0 log CFU/mL and Escherichia coli were from 5.3 log CFU/mL of initial state to 4.7 log CFU/mL to < <TEX>$10^1$</TEX> CFU/mL. The soluble solids and free sugars did not significantly differ (p<0.05) depending on conditions of PEF treatment. The total phenolic contents and antioxidant activity such as the DPPH and ferric reducing antioxidant power (FRAP) by PEF treatments were significantly partly reduced, but the PEF-reduced value came in smaller quantities than the heat treatment at <TEX>$65^{\circ}C$</TEX>. The iterative PEF treatments with pulse width of 25 <TEX>${\mu}s$</TEX> and pulse frequency of 85 Hz at electric field intensity of 20.0 kV/cm showed limited in microbial reduction. Also, total phenolic contents and antioxidant activity such as DPPH and FRAP, significantly decreased depending on treatment numbers of PEF (p<0.05).
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