Abstract
We used hybridoma technology to obtain 15 monoclonal antibodies (mAbs) to antigens of whole-cell suspension of M. avium. The isotypes and specificity of mAbs to mycobacterial and bacterial antigens were characterized. The chemical nature of the antigens/antigenic determinant recognized by the mAbs under treatment with proteinase K and metaperiodate Na and using extracted with ethanol and benzene M. avium suspension was investigated. All mAbs were purified by affinity chromatography and used in heterologous indirect two-site ELISA to determine diagnostic properties in the study of IgG antibodies in sera of patients with pulmonary tuberculosis, pulmonary mycobacteriosis or healthy donors. The presented method makes it possible to individually identify and take into account non-specific “natural” IgG antibodies of each serum under study (patent for invention No. 2794855). mAbs 1B11 showed diagnostic specificity in the detection of mycobacteriosis − 62.47% and sensitivity – 74.78%, 3E12 (anti-54kDa) − 88.89% and 52.63%, and 1H7G6 − 66.81% and 83.80%, respectively.
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